Localization of tektin filaments in microtubules of sea urchin sperm flagella by immunoelectron microscopy.
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چکیده
منابع مشابه
Localization of tektin filaments in microtubules of sea urchin sperm flagella by immunoelectron microscopy
Extraction of doublet microtubules from the sperm flagella of the sea urchin Strongylocentrotus purpuratus with sarkosyl (0.5%)-urea (2.5 M) yields a highly pure preparation of "tektin" filaments that we have previously shown to resemble intermediate filament proteins. They form filaments 2-3 nm in diameter as seen by negative stain electron microscopy and are composed of approximately equal am...
متن کاملStarting transients in sea urchin sperm flagella
Live sea urchin spermatozoa were rendered immotile by lowered pH; Triton-extracted spermatozoa were rendered immotile by either lowered pH or by deprivation of ATP. The spermatozoa began to beat after an increase in pH or as ATP was supplied, and the first bends were recorded on ciné film. Triton-extracted spermatozoa deprived of ATP retained a partially formed basal bend which could be either ...
متن کاملMovement of sea urchin sperm flagella
The motion of the sea urchin sperm flagellum was analyzed from high-speed cinemicrographs. At all locations on the flagellum the transversal motion and the curvature were found to vary sinusoidally in time. The curvatures of the flagella increase strongly near the proximal junction. Two sperm are described in transient from rest to normal motion. The full wave motion developed in both sperm wit...
متن کاملInhibition and relaxation of sea urchin sperm flagella by vanadate
Direct measurements of the stiffness (elastic bending resistance) of demembranated sera urchin sperm flagella were made in the presence of MgATP2- and vanadate. Under these conditions, the flagellum is in a relaxed state, with a stiffness of approximately 0.9 x 10(-21) N m2, which is approximately 5% of the stiffness obtained in the rigor state in the absence of MgATP2-. MgADP- dose not substit...
متن کاملConformational change in the outer doublet microtubules from sea urchin sperm flagella
Dark-field microscopy with a high-powered light source revealed that the outer doublet microtubules (DMTs) from sea urchin (Pseudocentrotus depressus and Hemicentrotus pulcherrimus) sperm flagella assume helically coiled configurations (Miki-Noumura, T., and R. Kamiya. 1976. Exp. Cell Res. 97: 451.). We report here that the DMTs change shape when the pH or Ca-ion concentration is changed. The D...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1985
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.100.1.126